Today we received our PCR samples and we have been assigned to test whether the PCR worked or not by using Gel Electrophoresis.
A blue dye was added to our DNA samples, before entering the well, to increase the viscosity of the sample. It will also make the process of electrophoresis visible. Then, the mixture was placed into the well through a pipet.
The amplified DNA samples we made using PCR have a negative charge, and at each extreme of the Gel Electrophoresis there is either a positive or negative charge. Our DNA samples are negatively charged and through the electric current forces exerted on the DNA, it should move towards the positive end, separating by weight.
The smaller and lighter strands will migrate faster to the positive end than the larger strands. This difference in migration will organize the strands by the size.
The Gel Electrophoresis will test for different DNA strands that were copied. By nature of the PCR, there should be only one band of DNA, all of the same size from the negative end to the positive end.
After performing the Gel Electrophoresis, we also inoculated yeast in 5mL of YPD broth using a pipette. We did this by creating a sterile environment with ethanol burners. We then used the pipette to transfer the yeast cells to the growth media in an eppendorf tube. The yeast was then grown for 5 days.