Today we had our second presentation where we expanded upon our hypothesis. Through research and performing the PCR and Gel Electrophoresis we now have a clearer idea of our experiment. A major emphasis throughout our presentation was how we planned to set-up the experiment.
1st Strand: Both SAK1 and TOS3
2nd Strand:TOS3 knocked out
3rd Strand:SAK1 knocked out.
We expect the strand with SAK1 to be the most productive under pH stress.
We will measure colony growth by running an assay with the strains of yeast.
We will have multiple intervals of pH ranging from 2-6 and we will be able to record the data on a line graph to compare the productivity of the strands.
We also answered a couple of practice questions that tested our knowledge about microbial growth in batch culture. The worksheet involved defining Yeast extract,Peptone, Dextrose, and Agar (nutritional and growth components in yeast fermentation). As well as the mathematical and chemical components involved in fermentation and yeast growth.
Finally, we received our yeast and observed that it had grown. Then, we performed a serial dilution as the first step to be able to measure yeast growth. A 1:1 ratio was maintained as we used 100 micro liters of sterile water and 100 micro liters of yeast culture. The pipette was used to make sure the 1:1 ratio was constant as it was transferred throughout the first row of wells. We observed how there were different shades of liquid throughout the wells.
That’s all for today, until next time!
Link to Presentation: https: lab-presentation-2