Lab Post #7 (9/19/16)

The goal of lab today was to created the YPD media for our yeast cells to grown and thrive on. YPD is a nutrient rich media that can be used in liquid form or used with agar.

The protocol for to create one liter of YPD for the whole class consists of:

Dextrose – 20g (Simple sugar; used as energy source for cell division and respiration)

Peptone – 20g (Short amino acid proteins; used for nutrition)

Yeast extract – 10g (The cell contests of Yeast; used for nutrition)

Distilled water – Filled to 1L

img_1843

This YPD was combined with 2 grams of agar and then put into an autoclave for sterilization. An autoclave is a machine that puts a sample under high pressure and heat to sterilize it.img_1850

A serial dilution in a well plate of our SAK1 deficient yeast culture was conducted 11 times with a 1:1 dilution for each time.  A serial dilution is a series compounding dilutions (each subsequent dilution is taken from the dilution before). This allows for an exponential increase in dilution over each time.

img_1849

The serial dilution was put into a spectrophotometer to find the concentration of yeast per each of the dilutions. A spectrophotometer is a device that calculates the transmittance of light through a medium which can therefore be used to calculate the concentration of the substance in the medium. The concentrations resulted as follows:

image

 

Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s