Lab Post #13 (10/10/16)

Today is the first day of the Chemistry Lab. We must recreate the Yeast (Saccharomyces Cerevisiae) experiment, but this time we plan on creating, through 3D printing, multiple open-ended manometers that will measure metabolic rates of anaerobic fermentation. Inside the container there will be the inoculated YPD (Wild type, TOS3 Deficient, or SAK1 Deficient) it will metabolize and produce carbon dioxide as a byproduct.Ethanol will also be placed in the manometer and will settle. The air pressure (carbon dioxide) will force the ethanol out of its initial position and then we can measure the change of air pressure. This information will let us measure metabolic rate over a certain period of time. We also plan on solidifying the gel with the appropriate pH in the wax paper. The wax paper will be designed in a star-shape. With the inoculated YPD in the center and the pH levels branched out from the center. The growth of the yeast will differ depending on the pH level branch (2-9 increments of 1). That is our plan.screen-shot-2016-10-10-at-4-31-22-pm

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